Abdul Basit Shaikh

Education

PhD in Drug Discovery – Universita Degli Studi Di Genova – Istituto Italiano Di Tecnologia, Genoa, Italy

Master of Science in Pharmaceutical Biotechnology – National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, India

Bachelor in Pharmaceutical Sciences – NDMVP Samaja’s College of Pharmacy, University of Pune, India

Licensure

Registered Pharmacist (India)

Research interests

• Discovery of endogenous probe for drug metabolizing enzymes to predict the interindividual variability and drug-drug interaction.
• Quantitative proteomic analysis of drug metabolizing enzyme (DMEs) and drug transporters to explore interindividual variability in DMEs and transporters expression in various tissue across preclinical species and human.
• In vitro drug metabolism and identification of metabolites using in vitro tools

About

Dr. Basit is an assistant research professor in the Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University Spokane, WA. He has worked as senior fellow and technical director for quantitative proteomics and metabolomics at Prasad Lab in the Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA. He also has working experience with in vitro DMPK in at Sailifesciences and Vanta Bioscience, India. Dr. Basit has published > 25 peer-reviewed articles and >30 conference abstracts.

His current research focus is biomarker discovery for drug metabolizing enzymes and drug transporters using cutting edge mass spectrometry platform for prediction of drug-drug interaction, adverse therapeutic events associated with narrow therapeutic window drug and prediction of precision dosing based on biomarkers.

Patents filed

IP: 48585.01US1 – “A novel LC-MS/MS method for PIVKAII quantification: Characterization of warfarin induced changes in prothrombin des-carboxylation”
Inventors:  Bhagwat Prasad, Kenneth E. Thummel, Abdul Basit, Allan E. Rettie

IP: 47911.02US1 – METHODS FOR TREATING TESTOSTERONE DEFICIENCY IN MEN AND METHODS FOR PRECISE DOSING OF UGT2B17 SUBSTRATE DRUGS”
Inventors:  Bhagwat Prasad, Abdul Basit, Haeyoung Zhang, John Amory

Selected publications

Basit, A.; Prasad, B.; Estergreen, J. K.; Sabath, D. E.; Alade, N.; Veenstra, D. L.; Rettie, A. E.; Thummel, K. E.  A Novel LC-MS/MS Assay for Quantification of Des-carboxy Prothrombin and Characterization of Warfarin-Induced Changes. Clin Transl Sci 2020.

Zhang, H.; Basit, A.; Wolford, C.; Chen, K. F.; Gaedigk, A.; Lin, Y. S.; Leeder, J. S.; Prasad, B.  Normalized Testosterone Glucuronide as a Potential Urinary Biomarker for Highly Variable UGT2B17 in Children 7-18 Years. Clin Pharmacol Ther 2020, 107, (5), 1149-1158.

Zhang, H.; Wolford, C.; Basit, A.; Li, A. P.; Fan, P. W.; Takahashi, R. H.; Khojasteh, S. C.; Smith, B. J.; Murray, B. P.; Thummel, K.; Prasad, B.  Regional proteomic quantification of clinically relevant non-cytochrome P450 enzymes along the human small intestine. Drug Metab Dispos 2020.

Basit, A^#.; Vozella^#, V.; Piras, F.; Realini, N.; Armirotti, A.; Bossu, P.; Assogna, F.; Sensi, S. L.; Spalletta, G.; Piomelli, D.  Elevated plasma ceramide levels in post-menopausal women: a cross-sectional study. Aging (Albany NY) 2019, 11, (1), 73-88. ^#Equal contribution first author.

Li, C. Y.; Hosey-Cojocari, C.; Basit, A.; Unadkat, J. D.; Leeder, J. S.; Prasad, B.  Optimized Renal Transporter Quantification by Using Aquaporin 1 and Aquaporin 2 as Anatomical Markers: Application in Characterizing the Ontogeny of Renal Transporters and Its Correlation with Hepatic Transporters in Paired Human Samples. AAPS J 2019, 21, (5), 88.

Li, C. Y.; Basit, A.; Gupta, A.; Gaborik, Z.; Kis, E.; Prasad, B.  Major glucuronide metabolites of testosterone are primarily transported by MRP2 and MRP3 in human liver, intestine and kidney. J Steroid Biochem Mol Biol 2019, 191, 105350.

Basit, A^#.; Hamid, Z^#.; Pontis, S.; Piras, F.; Assogna, F.; Bossu, P.; Pontieri, F. E.; Stefani, A.; Spalletta, G.; Franceschi, P.; Reggiani, A.; Armirotti, A.  Gender specific decrease of a set of circulating N-acylphosphatidyl ethanolamines (NAPEs) in the plasma of Parkinson’s disease patients. Metabolomics 2019, 15, (5), 74. ^#Equal contribution first author.

De Vita, T.; Albani, C.; Realini, N.; Migliore, M.; Basit, A.; Ottonello, G.; Cavalli, A.  Inhibition of Serine Palmitoyltransferase by a Small Organic Molecule Promotes Neuronal Survival after Astrocyte Amyloid Beta 1-42 Injury. ACS Chem Neurosci 2019, 10, (3), 1627-1635.

Bhatt, D. K.; Mehrotra, A.; Gaedigk, A.; Chapa, R.; Basit, A.; Zhang, H.; Choudhari, P.; Boberg, M.; Pearce, R. E.; Gaedigk, R.; Broeckel, U.; Leeder, J. S.; Prasad, B.  Age- and Genotype-Dependent Variability in the Protein Abundance and Activity of Six Major Uridine Diphosphate-Glucuronosyltransferases in Human Liver. Clin Pharmacol Ther 2019, 105, (1), 131-141.

Basit, A.; Radi, Z.; Vaidya, V. S.; Karasu, M.; Prasad, B.  Kidney Cortical Transporter Expression across Species Using Quantitative Proteomics. Drug Metab Dispos 2019, 47, (8), 802-808.

Zhang, H.; Basit, A.; Busch, D.; Yabut, K.; Bhatt, D. K.; Drozdzik, M.; Ostrowski, M.; Li, A.; Collins, C.; Oswald, S.; Prasad, B.  Quantitative characterization of UDP-glucuronosyltransferase 2B17 in human liver and intestine and its role in testosterone first-pass metabolism. Biochem Pharmacol 2018, 156, 32-42.

Basit, A^#.; Merrill, C. B^#.; Armirotti, A.; Jia, Y.; Gall, C. M.; Lynch, G.; Piomelli, D.  Author Correction: Patch clamp-assisted single neuron lipidomics. Sci Rep 2018, 8, (1), 6772. ^#Equal contribution first author.

Bhatt, D. K.; Basit, A.; Zhang, H.; Gaedigk, A.; Lee, S. B.; Claw, K. G.; Mehrotra, A.; Chaudhry, A. S.; Pearce, R. E.; Gaedigk, R.; Broeckel, U.; Thornton, T. A.; Nickerson, D. A.; Schuetz, E. G.; Amory, J. K.; Leeder, J. S.; Prasad, B.  Hepatic Abundance and Activity of Androgen- and Drug-Metabolizing Enzyme UGT2B17 Are Associated with Genotype, Age, and Sex. Drug Metab Dispos 2018, 46, (6), 888-896.

Basit, A.; Amory, J. K.; Prasad, B.  Effect of Dose and 5alpha-Reductase Inhibition on the Circulating Testosterone Metabolite Profile of Men Administered Oral Testosterone. Clin Transl Sci 2018, 11, (5), 513-522.

Vozella, V.; Basit, A.; Misto, A.; Piomelli, D.  Age-dependent changes in nervonic acid-containing sphingolipids in mouse hippocampus. Biochim Biophys Acta Mol Cell Biol Lipids 2017, 1862, (12), 1502-1511.

Tran, T. N.; Athanassiou, A.; Basit, A.; Bayer, I. S.  Starch-based bio-elastomers functionalized with red beetroot natural antioxidant. Food Chem 2017, 216, 324-33.

Di Pardo, A.; Basit, A.; Armirotti, A.; Amico, E.; Castaldo, S.; Pepe, G.; Marracino, F.; Buttari, F.; Digilio, A. F.; Maglione, V.  De novo Synthesis of Sphingolipids Is Defective in Experimental Models of Huntington’s Disease. Front Neurosci 2017, 11, 698.

Di Pardo, A.; Amico, E.; Basit, A.; Armirotti, A.; Joshi, P.; Neely, M. D.; Vuono, R.; Castaldo, S.; Digilio, A. F.; Scalabri, F.; Pepe, G.; Elifani, F.; Madonna, M.; Jeong, S. K.; Park, B. M.; D’Esposito, M.; Bowman, A. B.; Barker, R. A.; Maglione, V.  Defective Sphingosine-1-phosphate metabolism is a druggable target in Huntington’s disease. Sci Rep 2017, 7, (1), 5280.

Capodivento, G.; Visigalli, D.; Garnero, M.; Fancellu, R.; Ferrara, M. D.; Basit, A.; Hamid, Z.; Pastore, V. P.; Garibaldi, S.; Armirotti, A.; Mancardi, G.; Serrati, C.; Capello, E.; Schenone, A.; Nobbio, L.  Sphingomyelin as a myelin biomarker in CSF of acquired demyelinating neuropathies. Sci Rep 2017, 7, (1), 7831.

Realini, N.; Palese, F.; Pizzirani, D.; Pontis, S.; Basit, A.; Bach, A.; Ganesan, A.; Piomelli, D.  Acid Ceramidase in Melanoma: EXPRESSION, LOCALIZATION, AND EFFECTS OF PHARMACOLOGICAL INHIBITION. J Biol Chem 2016, 291, (5), 2422-34.

Basit, A.; Pontis, S.; Piomelli, D.; Armirotti, A.  Ion mobility mass spectrometry enhances low-abundance species detection in untargeted lipidomics. Metabolomics 2016, 12, 50.

Dotsey, E. Y.; Jung, K. M.; Basit, A.; Wei, D.; Daglian, J.; Vacondio, F.; Armirotti, A.; Mor, M.; Piomelli, D.  Peroxide-Dependent MGL Sulfenylation Regulates 2-AG-Mediated Endocannabinoid Signaling in Brain Neurons. Chem Biol 2015, 22, (5), 619-28.

Basit, A.; Piomelli, D.; Armirotti, A.  Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS. Anal Bioanal Chem 2015, 407, (17), 5189-98.

Astarita, G.; Avanesian, A.; Grimaldi, B.; Realini, N.; Justinova, Z.; Panlilio, L. V.; Basit, A.; Goldberg, S. R.; Piomelli, D.  Methamphetamine accelerates cellular senescence through stimulation of de novo ceramide biosynthesis. PLoS One 2015, 10, (2), e0116961.

Armirotti, A.; Basit, A.; Realini, N.; Caltagirone, C.; Bossu, P.; Spalletta, G.; Piomelli, D.  Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: application to human subjects with neurodegenerative dementia. Anal Biochem 2014, 455, 48-54.

Basu, S.; Basit, A.; Ravindran, S.; Patel, V. B.; Vangala, S.; Patel, H.  Split calibration curve: an approach to avoid repeat analysis of the samples exceeding ULOQ. Bioanalysis 2012, 4, (19), 2375-89.

Singh, A.; Basit, A.; Banerjee, U. C.  Burkholderia cenocepacia: a new biocatalyst for efficient bioreduction of ezetimibe intermediate. J Ind Microbiol Biotechnol 2009, 36, (11), 1369-74.